English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  A dynamic view of cellular processes by in vivo fluorescence auto- and cross-correlation spectroscopy

Bacia, K., & Schwille, P. (2003). A dynamic view of cellular processes by in vivo fluorescence auto- and cross-correlation spectroscopy. Methods, 29(1), 74-85. Retrieved from http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WN5-47NF5HH-4-25&_cdi=6953&_user=38661&_pii=S1046202302002918&_orig=search&_coverDate=01%2F31%2F2003&_sk=999709998&view=c&wchp=dGLbVlW-zSkzk&md5=5494597ae35eaa649eef287596287144&ie=/sdarticle.pdf.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F1A5-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-20DE-B
Genre: Journal Article

Files

show Files
hide Files
:
17244.pdf (Publisher version), 0B
Name:
17244.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Bacia, K.1, Author              
Schwille, P.1, Author              
Affiliations:
1Research Group of Experimental Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578554              

Content

show
hide
Free keywords: fluorescence correlation spectroscopy; single-molecule analysis; in vivo; photobleaching; colocalization; confocal microscopy; fluorescence resonance energy transfer; fluorescence recovery after photobleaching
 Abstract: Fluorescence correlation spectroscopy (FCS) is becoming increasingly popular as a technique that aims at complementing live cell images with biophysical information. This article provides both a short overview over recent intracellular FCS applications and a practical guide for investigators, who are seeking to integrate FCS into live cell imaging to obtain information on particle mobility, local concentrations, and molecular interactions. A brief introduction to the principles of FCS is provided, particularly emphasizing practical aspects such as the choice of appropriate dyes and positioning of the measurement volume in the sample. Possibilities and limitations in extracting parameters from autocorrelation curves are discussed, and attention is drawn to potential artifacts, such as photobleaching and probe aggregation. The principle of dual- color cross-correlation is reviewed along with considerations for proper setup and adjustment. Practical implications of nonideal conditions including incomplete focus overlap and spectral crosstalk are considered. Recent examples of both auto- and cross-correlation applications demonstrate the potential of FCS for cell biology. (C) 2002 Elsevier Science (USA). All rights reserved.

Details

show
hide
Language(s): eng - English
 Dates: 2003-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Methods
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 29 (1) Sequence Number: - Start / End Page: 74 - 85 Identifier: -