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  Trypanosoma cruzi poly-zinc finger protein: a novel DNA/RNA-binding CCHC-zinc finger protein

Espinosa, J. M., Portal, D., Lobo, G. S., Pereira, C. A., Alonso, G. D., Gomez, E. B., et al. (2003). Trypanosoma cruzi poly-zinc finger protein: a novel DNA/RNA-binding CCHC-zinc finger protein. Molecular and Biochemical Parasitology, 131(1): doi: 10.1016/S0166-6851(03)00187-7, pp. 35-44. Retrieved from http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T29-497H5CF-2-S&_cdi=4913&_user=38661&_pii=S0166685103001877&_orig=search&_coverDate=09%2F30%2F2003&_sk=998689998&view=c&wchp=dGLbVtz-zSkWb&md5=50ce60a05cb5b3a48bbbea57145b7886&ie=/sdarticle.pdf.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F1E0-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-E3FC-9
Genre: Journal Article

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 Creators:
Espinosa, J. M., Author
Portal, D., Author
Lobo, G. S., Author
Pereira, C. A., Author
Alonso, G. D., Author
Gomez, E. B., Author
Humbert-Lan, G.1, Author              
Rivera-Pomar, R.2, Author
Flawia, M. M., Author
Torres, H. N., Author
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              
2Max Planck Society, ou_persistent13              

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Free keywords: Trypanosoma cruzi; CCHC-type zinc finger; Poly-zinc finger protein; TcPZFP1; Serine–arginine-rich protein; TcSR
 Abstract: A poly-zinc finger protein, designated PZFP1 was identified in Trypanosoma cruzi for the first time. The protein has 191 amino acids, contains seven motifs Cys(X)2Cys(X)4His(X)4Cys. A recombinant PZFP1 was generated in E. coli and the expected 21 kDa polypeptide co-purified with two other inducible products of about 42 and 63 kDa.Western blot analysis of cell extracts using an anti-PZFP1 antibody recognized a major band of 41 kDa. Electrophoretic mobility shift analysis demonstrated that both, recombinant and native PZFP1, specifically interact with single-stranded DNA or RNA oligonucleotides carrying recognition sequences of other CCHC proteins. The protein was localized mainly in the cytoplasm and nucleus as observed by indirect immunofluorescence analysis. PZFP1 interacted specifically with a T. cruzi serine–arginine-rich protein (TcSR) in a yeast two-hybrid assay, suggesting a role in pre-mRNA processing.

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Language(s): eng - English
 Dates: 2004-04-142003
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: Peer
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Title: Molecular and Biochemical Parasitology
Source Genre: Journal
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Pages: - Volume / Issue: 131 (1) Sequence Number: doi: 10.1016/S0166-6851(03)00187-7 Start / End Page: 35 - 44 Identifier: -