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  Production of maternal-zygotic mutant zebrafish by germ-line replacement

Ciruna, B., Weidinger, G., Knaut, H., Thisse, B., Thisse, C., Raz, E., et al. (2002). Production of maternal-zygotic mutant zebrafish by germ-line replacement. Proceedings of the National Academy of Sciences of the United States of America, 99(23), 14919-14924. Retrieved from http://www.pnas.org/content/99/23/14919.full.pdf+html.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F295-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-816F-0
Genre: Journal Article

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599445.pdf (Publisher version), 212KB
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Ciruna, B., Author
Weidinger, G.1, Author              
Knaut, H., Author
Thisse, B., Author
Thisse, C., Author
Raz, E.1, Author              
Schier, A. F., Author
Affiliations:
1Research Group of Germ Cell Development, MPI for biophysical chemistry, Max Planck Society, ou_578603              

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 Abstract: We report a generally applicable strategy for transferring zygotic lethal mutations through the zebrafish germ line. By using a morpholino oligonucleotide that blocks primordial germ cell (PGC) development, we generate embryos devoid of endogenous PGCs to serve as hosts for the transplantation of germ cells derived from homozygous mutant donors. Successful transfers are identified by the localization of specifically labeled donor PGCs to the region of the developing gonad in chimeric embryos. This strategy, which results in the complete replacement of the host germ line with donor PGCs, was validated by the generation of maternal and maternal-zygotic mutants for the miles apart locus. This germ-line replacement technique provides a powerful tool for studying the maternal effects of zygotic lethal mutations. Furthermore, the ability to generate large clutches of purely mutant embryos will greatly facilitate embryological, genetic, genomic, and biochemical studies.

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Language(s): eng - English
 Dates: 2002-11-12
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: Proceedings of the National Academy of Sciences of the United States of America
Source Genre: Journal
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Pages: - Volume / Issue: 99 (23) Sequence Number: - Start / End Page: 14919 - 14924 Identifier: -