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  The mitotic-spindle-associated protein astrin is essential for progression through mitosis

Gruber, J., Harborth, J., Schnabel, J., Weber, K., & Hatzfeld, M. (2002). The mitotic-spindle-associated protein astrin is essential for progression through mitosis. Journal of Cell Science, 115(21), 4053-4059. Retrieved from http://jcs.biologists.org/cgi/reprint/115/21/4053.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F29B-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-9D11-A
Genre: Journal Article

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599448.pdf (Publisher version), 419KB
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 Creators:
Gruber, J.1, Author              
Harborth, J.1, Author              
Schnabel, J.1, Author              
Weber, K.1, Author              
Hatzfeld, M.1, Author              
Affiliations:
1Department of Biochemistry and Cell Biology, MPI for biophysical chemistry, Max Planck Society, ou_578618              

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Free keywords: astrin; coiled coil; microtubules; mitotic spindle; RNA; interference
 Abstract: Astrin is a mitotic-spindle-associated protein expressed in most human cell lines and tissues. However, its functions in spindle organization and mitosis have not yet been determined. Sequence analysis revealed that astrin has an N-terminal globular domain and an extended coiled-coil domain. Recombinant astrin was purified and characterized by CD spectroscopy and electron microscopy. Astrin showed parallel dimers with head- stalk structures reminiscent of motor proteins, although no sequence similarities to known motor proteins were found. In physiological buffers, astrin dimers oligomerized via their globular head domains and formed aster-like structures. Silencing of astrin in HeLa cells by RNA interference resulted in growth arrest, with formation of multipolar and highly disordered spindles. Chromosomes did not congress to the spindle equator and remained dispersed. Cells depleted of astrin were normal during interphase but were unable to progress through mitosis and finally ended in apoptotic cell death. Possible functions of astrin in mitotic spindle organization are discussed.

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Language(s): eng - English
 Dates: 2002-11-01
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: Journal of Cell Science
Source Genre: Journal
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Pages: - Volume / Issue: 115 (21) Sequence Number: - Start / End Page: 4053 - 4059 Identifier: -