English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Pax6 is required for the normal development of the forebrain axonal connections

Jones, L., Lopez-Bendito, G., Gruss, P., Stoykova, A., & Molnar, Z. (2002). Pax6 is required for the normal development of the forebrain axonal connections. Development, 129(21), 5041-5052. Retrieved from http://dev.biologists.org/content/129/21/5041.full.pdf+html.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F2A4-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-E49E-8
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Jones, L., Author
Lopez-Bendito, G., Author
Gruss, P.1, Author              
Stoykova, A.2, Author              
Molnar, Z., Author
Affiliations:
1Department of Molecular Cell Biology, MPI for biophysical chemistry, Max Planck Society, ou_578585              
2Research Group of Molecular Developmental Neurobiology, MPI for biophysical chemistry, Max Planck Society, ou_578587              

Content

show
hide
Free keywords: axon guidance; patterning; telencephalon; semaphorins; mouse; pallial/subpallial boundary; thalamocortical projections; corticofugal axons; mouse
 Abstract: The transcription factor PAX6 has been implicated in forebrain patterning, cerebral cortical arealization and in development of thalamocortical connections. Using a Pax6/lacZ knockout mouse, in which the endogenous Pax6 expression is reflected by beta-galactosidase activity, we have studied the consequences of the loss of Pax6 function on thalamocortical (TCA) and corticofugal axon (CIA) pathfinding during the period of embryonic day (E) 14.5 to E18.5. Carbocyanine dye tracing in Pax6 heterozygotes (Pax6(+/-)) and Pax6 wild-type (Pax6(+/+)) brains revealed that CFAs and TCAs temporarily arrested their growth at E14.5 at the border of the P-galactosidase-positive region at the pallial/subpallial boundary (PSPB), before they continued towards their targets. However, in Pax6 homozygous (Pax6(-/-)) embryos, CFAs and TCAs were unable to encounter each other at the PSPB and reach their final targets. Instead of crossing the PSPB, they had the tendency to descend into the ventral pallium in large aberrant fascicles. In addition, cells with a presumptive guide-post function, which are normally situated in the ventral thalamus, internal capsule and hypothalamus, were more dispersed in the hypothalamus and ventral pallium. These pathfinding defects were confirmed by immunohistochemistry for L1 and TAG1, markers of the early axonal connections. The aberrant development of axonal connections in absence of Pax6 function appear to be related to ultrastructural defects of cells along the PSPB, as well as to a failure of axonal guidance molecule expression, including Sema3c and Sema5a.

Details

show
hide
Language(s): eng - English
 Dates: 2002-11
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Development
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 129 (21) Sequence Number: - Start / End Page: 5041 - 5052 Identifier: -