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  Specific interaction of p53 with target binding sites is determined by DNA conformation and is regulated by the C- terminal domain

Goehler, T., Reimann, M., Cherny, D. I., Walter, K., Warnecke, G., Kim, E., et al. (2002). Specific interaction of p53 with target binding sites is determined by DNA conformation and is regulated by the C- terminal domain. Journal of Biological Chemistry, 277(43), 41192-41203. Retrieved from http://www.jbc.org/content/277/43/41192.full.pdf+html.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F2B3-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-E236-F
Genre: Journal Article

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599462.pdf (Publisher version), 958KB
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 Creators:
Goehler, T., Author
Reimann, M., Author
Cherny, D. I.1, Author              
Walter, K.2, Author              
Warnecke, G., Author
Kim, E., Author
Deppert, W., Author
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              
2Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

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 Abstract: Transcriptional activation of p53-regulated genes is initiated by sequence-specific DNA binding of p53 to target binding sites. Regulation of sequence-specific DNA binding is complex and occurs at various levels. We demonstrate that DNA topology is an important parameter for regulating the selective and highly specific interaction of p53 with its target binding sites. Specific binding of wild-type p53 is greatly enhanced when cognate binding sites are present in a non-linear stem- loop conformation. The C-terminal domain plays a key role in regulating the specific interactions of p53 with target binding sites in a DNA conformation-dependent manner. The C-terminal domain is required for binding to target sites in a non-linear DNA conformation in contrast to the strong inhibitory effects of the C terminus on p53 interaction with linear DNA. We propose that selective binding of p53 to various promoters may be determined by the DNA conformation within p53 cognate sites.

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Language(s): eng - English
 Dates: 2002-10-25
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: Journal of Biological Chemistry
Source Genre: Journal
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Pages: - Volume / Issue: 277 (43) Sequence Number: - Start / End Page: 41192 - 41203 Identifier: -