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  The N-terminal domains of syntaxin 7 and vti1b form three-helix bundles that differ in their ability to regulate SNARE complex assembly

Antonin, W., Dulubova, I., Arac, D., Pabst, S., Plitzner, J., Rizo, J., et al. (2002). The N-terminal domains of syntaxin 7 and vti1b form three-helix bundles that differ in their ability to regulate SNARE complex assembly. Journal of Biological Chemistry, 277(39), 36449-36456. Retrieved from http://www.jbc.org/content/277/39/36449.full.pdf+html.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F2E6-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-E238-B
Genre: Journal Article

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599490.pdf (Publisher version), 972KB
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Antonin, W.1, Author              
Dulubova, I., Author
Arac, D., Author
Pabst, S.1, Author              
Plitzner, J.1, Author              
Rizo, J., Author
Jahn, R.1, Author              
Affiliations:
1Department of Neurobiology, MPI for biophysical chemistry, Max Planck Society, ou_578595              

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 Abstract: The SNAREs syntaxin 7, syntaxin 8, vti1b, and endobrevin/VAMP8 function in the fusion of late endosomes. Although the core complex formed by these SNAREs is very similar to the neuronal SNARE complex, it differs from the neuronal complex in that three of the four SNAREs contain extended N-terminal regions of unknown structure and function. Here we show that the N- terminal regions of syntaxin 7, syntaxin 8, and vti1b contain well folded a-helical domains. Multidimensional NAIR spectroscopy revealed that in syntaxin 7 and vti1b, the domains form three-helix bundles resembling those of syntaxin 1, Sso1p, and Vam3p. The three-helix bundle domain of vti1b is the first of its kind identified in a SNARE outside the syntaxin family. Only syntaxin 7 adopts a closed conformation, whereas in vti1b and syntaxin 8, the N-terminal domains do not interact with the adjacent SNARE motifs. Accordingly, the rate of SNARE complex assembly is retarded about 7-fold when syntaxin 7 contains its N-terminal domain, whereas the N-terminal domains of vti1b and syntaxin 8 have no influence on assembly kinetics. We conclude that three-helix bundles represent a common fold for SNARE N- terminal domains, not restricted to the syntaxin family. However, they differ in their ability to adopt closed conformations and thus to regulate the assembly of SNARE complexes.

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Language(s): eng - English
 Dates: 2002-09-27
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: Journal of Biological Chemistry
Source Genre: Journal
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Pages: - Volume / Issue: 277 (39) Sequence Number: - Start / End Page: 36449 - 36456 Identifier: -