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  Effect of cholesterol on the interaction of seminal plasma protein, PDC-109 with phosphatidylcholine membranes

Swamy, M. J., Marsh, D., Anbazhagan, V., & Ramakrishnan, M. (2002). Effect of cholesterol on the interaction of seminal plasma protein, PDC-109 with phosphatidylcholine membranes. FEBS Letters, 528(1-3), 230-234. Retrieved from http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T36-46NX63R-9-1&_cdi=4938&_user=38661&_pii=S0014579302033161&_origin=search&_coverDate=09%2F25%2F2002&_sk=994719998&view=c&wchp=dGLzVzz-zSkWb&md5=8a56309f2fa4f8b947ad2f1c6c79b080&ie=/sdarticle.pdf.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F2ED-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-E4AA-A
Genre: Journal Article

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16665.pdf (Publisher version), 0B
 
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Swamy, M. J.1, Author              
Marsh, D.1, Author              
Anbazhagan, V., Author
Ramakrishnan, M.1, Author              
Affiliations:
1Department of Spectroscopy and Photochemical Kinetics, MPI for biophysical chemistry, Max Planck Society, ou_578624              

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Free keywords: BSP-Al; lipid-protein interaction; lipid bilayer; cholesterol efflux; phosphatidylcholine; sphingomyelin
 Abstract: Binding of PDC-109, the major protein of the bovine seminal plasma, to sperm plasma membrane results in an efflux of cholesterol and choline phospholipids, a necessary event before capacitation can occur. The selectivity of PDC-109 for different spin-labelled phospholipids and sterol probes in dimyristoylphosphatidylcholine (DMPC) host matrix has been characterized earlier by EPR spectroscopy [Ramakrishnan, M., Anbazhagan, V., Pratap, T.V., Marsh, D. and Swamy, M.J. (2001) Biophys. J. 81, 2215-2225]. In this report the effect of cholesterol on the interaction of PDC-109 with DMPC membranes has been investigated by spin-label EPR spectroscopy. The results indicate that the presence of cholesterol leads to an increased association of different phospholipid as well as sterol probes, thus modulating the interaction of PDC-109 with phospholipid membranes. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

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Language(s): eng - English
 Dates: 2002-09-25
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: FEBS Letters
Source Genre: Journal
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Pages: - Volume / Issue: 528 (1-3) Sequence Number: - Start / End Page: 230 - 234 Identifier: -