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  Single-stranded antisense siRNAs guide target RNA cleavage in RNAi

Martinez, J., Patkaniowska, A., Urlaub, H., Luehrmann, R., & Tuschl, T. (2002). Single-stranded antisense siRNAs guide target RNA cleavage in RNAi. Cell, 110(5), 563-574. Retrieved from http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WSN-4C5HD56-5-1&_cdi=7051&_user=38661&_pii=S009286740200908X&_origin=search&_coverDate=09%2F06%2F2002&_sk=998899994&view=c&wchp=dGLbVlb-zSkzV&md5=e60d5b61ed34db921ee5f181faeafa4b&ie=/sdarticle.pdf.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F2FE-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-9D13-6
Genre: Journal Article

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 Creators:
Martinez, J., Author
Patkaniowska, A., Author
Urlaub, H.1, Author              
Luehrmann, R.2, Author              
Tuschl, T.3, Author              
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              
2Department of Cellular Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578576              
3Research Group of Combinatorical Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578556              

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 Abstract: Small interfering RNAs (siRNAs) are the mediators of mRNA degradation in the process of RNA interference (RNAi). Here, we describe a human biochemical system that recapitulates siRNA- mediated target RNA degradation. By using affinity-tagged siRNAs, we demonstrate that a single-stranded siRNA resides in the RNA-induced silencing complex (RISC) together with eIF2C1 and/or eIF2C2 (human GERp95) Argonaute proteins. RISC is rapidly formed in HeLa cell cytoplasmic extract supplemented with 21 nt siRNA duplexes, but also by adding single-stranded antisense RNAs, which range in size between 19 and 29 nucleotides. Single-stranded antisense siRNAs are also effectively silencing genes in HeLa cells, especially when 5'- phosphorylated, and expand the repertoire of RNA reagents suitable for gene targeting.

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Language(s): eng - English
 Dates: 2002-09-06
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: Cell
Source Genre: Journal
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Pages: - Volume / Issue: 110 (5) Sequence Number: - Start / End Page: 563 - 574 Identifier: -