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  Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules

Riedel, D., Antonin, W., Fernandez-Chacon, R., de Toledo, G. A., Jo, T., Geppert, M., et al. (2002). Rab3D is not required for exocrine exocytosis but for maintenance of normally sized secretory granules. Molecular and Cellular Biology, 22(18), 6487-6497. Retrieved from http://mcb.asm.org/cgi/reprint/22/18/6487.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F30F-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-BCA3-4
Genre: Journal Article

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 Creators:
Riedel, D.1, Author              
Antonin, W.2, Author              
Fernandez-Chacon, R.3, Author              
de Toledo, G. A., Author
Jo, T., Author
Geppert, M., Author
Valentijn, J. A., Author
Valentijn, K., Author
Jamieson, J. D., Author
Suedhof, T. C., Author
Jahn, R.2, Author              
Affiliations:
1Facility for Electron Microscopy, MPI for biophysical chemistry, Max Planck Society, ou_578615              
2Department of Neurobiology, MPI for biophysical chemistry, Max Planck Society, ou_578595              
3Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578579              

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 Abstract: Rab3D, a member of the Rab3 subfamily of the Rab/ypt GTPases, is expressed on zymogen granules in the pancreas as well as on secretory vesicles in mast cells and in the parotid gland. To shed light on the function of Rab3D, we have generated Rab3D- deficient mice. These mice are viable and have no obvious phenotypic changes. Secretion of mast cells is normal as revealed by capacitance patch clamping. Furthermore, enzyme content and overall morphology are unchanged in pancreatic and parotid acinar cells of knockout mice. Both the exocrine pancreas and the parotid gland show normal release kinetics in response to secretagogue stimulation, suggesting that Rab3D is not involved in exocytosis. However, the size of secretory granules in both the exocrine pancreas and the parotid gland is significantly increased, with the volume being doubled. We conclude that Rab3D exerts its function during granule maturation, possibly by preventing homotypic fusion of secretory granules.

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Language(s): eng - English
 Dates: 2002-09
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: Molecular and Cellular Biology
Source Genre: Journal
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Pages: - Volume / Issue: 22 (18) Sequence Number: - Start / End Page: 6487 - 6497 Identifier: -