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  Axial superresolution with ultrahigh aperture lenses

Blanca, C. M., & Hell, S. W. (2002). Axial superresolution with ultrahigh aperture lenses. Optics Express, 10(17), 893-898. Retrieved from http://www.opticsinfobase.org/oe/viewmedia.cfm?uri=oe-10-17-893&seq=0.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F32C-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-E461-E
Genre: Journal Article

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 Creators:
Blanca, C. M.1, Author
Hell, S. W.2, Author              
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2Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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 Abstract: We explore the current limits of the axial resolution of optical sectioning microscopy using a single lens, by combining the resolving power of novel 1.45 numerical aperture oil immersion lenses with superresolving binary aperture filters. We quantify the axial resolution brought about by the increase in semiaperture angle to alpha(max)=72.8degrees and demonstrate an absolute gain in axial resolution through binary pupil filters. Implemented in a confocalized two-photon excitation microscope, the combination of both effectively improves the axial resolution to 330 nm full-width-half-maximum, which is by 34% over that of a standard 1.4 NA system. (C) 2002 Optical Society of America

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Language(s): eng - English
 Dates: 2002-08-26
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: Optics Express
Source Genre: Journal
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Pages: - Volume / Issue: 10 (17) Sequence Number: - Start / End Page: 893 - 898 Identifier: -