English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Interaction of human serum albumin with membranes containing polymer-grafted lipids: spin-label ESR studies in the mushroom and brush regimes

Bartucci, R., Pantusa, M., Marsh, D., & Sportelli, L. (2002). Interaction of human serum albumin with membranes containing polymer-grafted lipids: spin-label ESR studies in the mushroom and brush regimes. Biochimica et Biophysica Acta-Biomembranes, 1564(1), 237-242. Retrieved from http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T1T-45TTW8V-5-G&_cdi=4899&_user=38661&_pii=S0005273602004583&_origin=search&_coverDate=08%2F19%2F2002&_sk=984359998&view=c&wchp=dGLzVlz-zSkzk&md5=1d4fa5a7e5813eadd2e91077e5b6fd18&ie=/sdarticle.pdf.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F331-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-E9E4-9
Genre: Journal Article

Files

show Files
hide Files
:
16507.pdf (Publisher version), 0B
Name:
16507.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Bartucci, R., Author
Pantusa, M., Author
Marsh, D.1, Author              
Sportelli, L., Author
Affiliations:
1Department of Spectroscopy and Photochemical Kinetics, MPI for biophysical chemistry, Max Planck Society, ou_578624              

Content

show
hide
Free keywords: polymer lipid; PEG-lipid; serum albumin; steric protection; opsonization; liposome; lipid-protein interaction; spin label; electron spin resonance
 Abstract: The adsorption of human serum albumin (HSA) to dipalmitoyl phosphatidylcholine (DPPC) bilayer membranes containing poly(ethylene glycol)-grafted dipalmitoyl phosphatidylethanolamine (PEG-DPPE) was studied as a function of content and headgroup size of the polymer lipid. In the absence of protein, conversion from the low-density mushroom regime to the high-density brush regime of polymer-lipid content is detected by the change in ESR outer hyperfine splitting, 2A(max), of chain spin-labelled phosphatidylcholine in gel-phase membranes. The values of 2A,; remain constant in the mushroom regime, but decrease on entering the brush regime. Conversion between the two regimes occurs at mole fractions X- PEG(m --> h) approximate to 0.04, 0.01-0.02 and 0.005-0.01 for PEG-DPPE with mean PEG molecular masses of 350, 2000 and 5000 Da, respectively, as expected theoretically. Adsorption of HSA to DPPC membranes is detected as a decrease of the spin label 2.4(max) hyperfine splitting in the gel phase. Saturation is obtained at a protein/lipid ratio of ca. 1:1 w/w. In the presence of polymer-grafted lipids, HSA adsorbs to DPPC membranes only in the mushroom regime, irrespective of polymer length. In the brush regime, the spin-label values of 2A(max) are unchanged in the presence of protein. Even in the mushroom regime, protein adsorption progressively becomes strongly attenuated as a result of the steric stabilization exerted by the polymer lipid. These results are in agreement with theoretical estimates of the lateral pressure exerted by the grafted polymer in the brush and mushroom regimes, respectively. (C) 2002 Elsevier Science B.V. All rights reserved.

Details

show
hide
Language(s): eng - English
 Dates: 2002-08-19
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Biochimica et Biophysica Acta-Biomembranes
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 1564 (1) Sequence Number: - Start / End Page: 237 - 242 Identifier: -