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  Oligomerization of DsRed is required for the generation of a functional red fluorescent chromophore

Sacchetti, A., Subramaniam, V., Jovin, T. M., & Alberti, S. (2002). Oligomerization of DsRed is required for the generation of a functional red fluorescent chromophore. FEBS Letters, 525(1-3), 13-19. Retrieved from http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T36-46872RD-4-H&_cdi=4938&_user=38661&_pii=S0014579302028740&_origin=search&_coverDate=08%2F14%2F2002&_sk=994749998&view=c&wchp=dGLbVlz-zSkzS&md5=24f6e9f8ff54be76b77bc6332b804b17&ie=/sdarticle.pdf.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F338-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-E441-5
Genre: Journal Article

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Sacchetti, A., Author
Subramaniam, V.1, Author              
Jovin, T. M.1, Author              
Alberti, S., Author
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1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

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Free keywords: green fluorescent protein; red fluorescent protein; chromophore; protein folding; fluorescent energy transfer
 Abstract: The coral red fluorescent protein (DsRed) absorbs and emits light at much higher wavelengths than the structurally homologous green fluorescent protein, raising questions about the properties of its chromophore. We have analyzed the relationship between the aggregation state and fluorescence of native, 6-histidine-tagged, or maltose-binding protein-fused DsRed. In all cases, newly synthesized DsRed molecules were largely monomeric and devoid of covalently closed chromophores. Maturation in vitro induces the expression of red fluorescent chromophores but only in oligomeric forms of the protein, whereas monomers are essentially devoid of fluorescence. NaOH- denatured samples demonstrated a generalized breakdown of the DsRed oligomers to monomers, which refolded after neutralization into weakly green fluorescent and still monomeric species. Red fluorescent chromophores were regenerated only upon oligomerization. These findings demonstrate that 'red' chromophores form and are functional only as oligomers, and suggest that the smallest red fluorescent functional unit is a dimer. A comparison of alkali- , acid- and guanidinium-denatared DsRed indicates that stabilization of the DsRed chromophore by concerted steps of folding and oligomerization may play a critical role in its maturation process. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

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Language(s): eng - English
 Dates: 2002-08-14
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: FEBS Letters
Source Genre: Journal
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Pages: - Volume / Issue: 525 (1-3) Sequence Number: - Start / End Page: 13 - 19 Identifier: -