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  Inwardly rectifying K+ (Kir) channels in Drosophila - A crucial role of cellular milieu factors for Kir channel function

Doering, F., Wischmeyer, E., Kuehnlein, R. P., Jaeckle, H., & Karschin, A. (2002). Inwardly rectifying K+ (Kir) channels in Drosophila - A crucial role of cellular milieu factors for Kir channel function. Journal of Biological Chemistry, 277(28), 25554-25561. Retrieved from http://www.jbc.org/content/277/28/25554.full.pdf+html.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F372-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-E217-6
Genre: Journal Article

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 Creators:
Doering, F.1, Author              
Wischmeyer, E.1, Author              
Kuehnlein, R. P.2, Author              
Jaeckle, H.3, Author              
Karschin, A.1, Author              
Affiliations:
1Research Group of Molecular Neurobiology of Signal Transduction, MPI for biophysical chemistry, Max Planck Society, ou_578561              
2Research Group of Molecular Physiology, MPI for biophysical chemistry, Max Planck Society, ou_578592              
3Department of Molecular Developmental Biology, MPI for biophysical chemistry, Max Planck Society, ou_578590              

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 Abstract: Three cDNAs encoding inwardly rectifying potassium (Kir) channels were isolated from Drosophila melanogaster. The protein sequences of Drosophila KirI (dKirI) and dKirII are moderately (<44%) and dKirIII sequence is weakly (<27%) identical to human Kir channel subunits. During fly development, five dKir channel transcripts derived from three genes are differentially expressed. Whole mount in situ hybridizations revealed dKirI transcripts absent from embryos, but dKirII and dKirIII are expressed in the embryonic hind gut and in Malpighian tubules, respectively, thus covering the entire osmoregulatory system of the developing fly. In the head of adult flies, predominantly dKirII transcripts were detected. When expressed in Xenopus oocytes, dKir channel activity was only observed after amino acid substitutions in their cytosolic tails (e.g. exchange of a unique valine in the NH2 terminus). In contrast, heterologous expression of wild type dKirI and dYirII in Drosophila S2 cells readily evoked typical inwardly rectifying K+ currents, which were weakly sensitive to Ba2+. Thus, the specific milieu of insect cells provides a crucial cellular environment for proper function of dKir channels.

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Language(s): eng - English
 Dates: 2002-07-12
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: Journal of Biological Chemistry
Source Genre: Journal
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Pages: - Volume / Issue: 277 (28) Sequence Number: - Start / End Page: 25554 - 25561 Identifier: -