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  Determination of the unknown phase difference in 4Pi-confocal microscopy through the image intensity

Blanca, C. M., Bewersdorf, J., & Hell, S. W. (2002). Determination of the unknown phase difference in 4Pi-confocal microscopy through the image intensity. Optics Communications, 206(4-6), 281-285. Retrieved from http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6TVF-45PTYPJ-7-1R&_cdi=5533&_user=38661&_pii=S0030401802014244&_origin=search&_coverDate=06%2F01%2F2002&_sk=997939995&view=c&wchp=dGLbVzz-zSkzk&md5=d5d1654fc9c5392efb239604d6ad7c28&ie=/sdarticle.pdf.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F399-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-E599-8
Genre: Journal Article

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16487.pdf (Publisher version), 0B
 
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 Creators:
Blanca, C. M., Author
Bewersdorf, J.1, Author              
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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 Abstract: We investigate the effect of the phase difference of the interfering wavefronts on the image intensity in 4Pi-confocal microscopy and thereby introduce a method to diminish the problem of the unknown phase difference in this microscope. An efficient implementation of our method utilizes binary amplitude filters reshaping the point-spread-function such that a significant disparity arises between the maximum fluorescence intensity in the constructive and destructive interference case. In the presence of planar and point-like features in the sample this property enables the system to unambiguously determine the mode of interference. (C) 2002 Elsevier Science B.V. All rights reserved

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Language(s): eng - English
 Dates: 2002-06-01
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: Optics Communications
Source Genre: Journal
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Pages: - Volume / Issue: 206 (4-6) Sequence Number: - Start / End Page: 281 - 285 Identifier: -