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  Evaluation of uncertainty in alignment tensors obtained from dipolar couplings

Zweckstetter, M., & Bax, A. (2002). Evaluation of uncertainty in alignment tensors obtained from dipolar couplings. Journal of Biomolecular NMR, 23(2), 127-137. Retrieved from http://springerlink.metapress.com/content/ptb0r768ce8fx375/fulltext.pdf.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F39F-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-DF6A-1
Genre: Journal Article

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Zweckstetter, M.1, Author              
Bax, A., Author
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1Research Group of Protein Structure Determination using NMR, MPI for biophysical chemistry, Max Planck Society, ou_578571              

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Free keywords: alignment tensor; backbone dynamics; dipolar coupling; error analysis; liquid crystal; order matrix; protein structure determination; ubiquitin
 Abstract: Residual dipolar couplings and their corresponding alignment tensors are useful for structural analysis of macromolecules. The error in an alignment tensor, derived from residual dipolar couplings on the basis of a known structure, is determined not only by the accuracy of the measured couplings but also by the uncertainty in the structure (structural noise). This dependence is evaluated quantitatively on the basis of simulated structures using Monte-Carlo type analyses. When large numbers of dipolar couplings are available, structural noise is found to result in a systematic underestimate of the magnitude of the alignment tensor. Particularly in cases where only few dipolar couplings are available, structural noise can cause significant errors in best-fitted alignment tensor values, making determination of the relative orientation of small fragments and evaluation of local backbone mobility from dipolar couplings difficult. An example for the protein ubiquitin demonstrates the inherent limitations in characterizing motions on the basis of local alignment tensor magnitudes.

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Language(s): eng - English
 Dates: 2002-06
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: Journal of Biomolecular NMR
Source Genre: Journal
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Pages: - Volume / Issue: 23 (2) Sequence Number: - Start / End Page: 127 - 137 Identifier: -