English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Cytoplasmic intermediate filament protein expression in tunicate development: a specific marker for the test cells

Wang, J., Karabinos, A., Zimek, A., Meyer, M., Riemer, D., Hudson, C., et al. (2002). Cytoplasmic intermediate filament protein expression in tunicate development: a specific marker for the test cells. European Journal of Cell Biology, 81(5), 302-311. Retrieved from http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B7GJ2-4DPMB1J-7B-1&_cdi=20194&_user=38661&_pii=S0171933504702332&_origin=search&_coverDate=05%2F01%2F2002&_sk=999189994&view=c&wchp=dGLzVtz-zSkzV&md5=7c8dc50e50496120d7d72c0810e0c808&ie=/sdarticle.pdf.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F3E2-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-9CDC-A
Genre: Journal Article

Files

show Files
hide Files
:
17086.pdf (Publisher version), 0B
Name:
17086.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Wang, J.1, Author              
Karabinos, A.1, Author              
Zimek, A.1, Author              
Meyer, M., Author
Riemer, D.1, Author              
Hudson, C., Author
Lemaire, P., Author
Weber, K.1, Author              
Affiliations:
1Department of Biochemistry and Cell Biology, MPI for biophysical chemistry, Max Planck Society, ou_578618              

Content

show
hide
Free keywords: Ciona intestinalis; intermediate filaments; keratins; test cells; urochordates
 Abstract: The urochordate Ciona intestinalis is a well established system for embryological studies, and large scale EST sequences begin to emerge. We cloned five cytoplasmic intermediate filament (IF) cDNAs and made specific antibodies to the recombinant proteins. Self-assembly studies and immunofluorescence microscopy were used to study these proteins and their distribution. Confirming and extending previous studies in Styela, we found that Ciona protein IF-A is expressed in muscle and forms homopolymeric filaments while proteins IF-C and IF-D, which form only obligatory heteropolymeric filaments, resemble a keratin pair exclusively found in the entire epidermis. Protein IF-B and the new protein IF-F potentially reflect tunicate-specific IF proteins. They are found in the entire internal epithelia including the neural gland. We also extended the analysis to earlier developmental stages of Ciona. Protein IF-A is expressed in muscle from larval stages, whereas proteins IF-C and IF-D are found only in the tail epidermis. Protein IF-F is detected abundantly in the test cells of eggs, embryos and premetamorphic larvae. Our studies show that IF proteins could prove very useful markers in the study of cell fate determination in Ciona. They also support previous findings on the evolutionary relationships of different IF proteins. Non-vertebrate chordates have IF proteins which represent orthologs of vertebrate type I to III proteins, but also IF proteins that do not seem to fit into these classes. However, the intron positions of all tunicate IF genes are conserved with vertebrate type I to III genes, pointing to a common evolutionary origin.

Details

show
hide
Language(s): eng - English
 Dates: 2002-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: European Journal of Cell Biology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 81 (5) Sequence Number: - Start / End Page: 302 - 311 Identifier: -