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  Focal spots of size lambda/23 open up far-field florescence microscopy at 33 nm axial resolution

Dyba, M., & Hell, S. W. (2002). Focal spots of size lambda/23 open up far-field florescence microscopy at 33 nm axial resolution. Physical Review Letters, 88(16), 163901-1-163901-4. Retrieved from http://prl.aps.org/pdf/PRL/v88/i16/e163901.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F3FA-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-8FF7-E
Genre: Journal Article

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Dyba, M.1, Author              
Hell, S. W.1, Author              
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1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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 Abstract: We report spots of excited molecules of 33 nm width created with focused light of lambda = 760 nm wavelength and conventional optics along the optic axis. This is accomplished by exciting the molecules with a femtosecond pulse and subsequent depletion of their excited state with red-shifted, picosecond-pulsed, counterpropagating, coherent light fields. The lambda/23 ratio constitutes what is to our knowledge the sharpest spatial definition attained with freely propagating electromagnetic radiation. The sub-diffraction spots enable for the first time far-field fluorescence microscopy with resolution at the tens of nanometer scale, as demonstrated in images of membranes of bacillus megaterium

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Language(s): eng - English
 Dates: 2002-04-22
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: Physical Review Letters
Source Genre: Journal
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Pages: - Volume / Issue: 88 (16) Sequence Number: - Start / End Page: 163901-1 - 163901-4 Identifier: -