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  Comparison of the axial resolution of practical Nipkow-disk confocal fluorescence microscopy with that of multifocal multiphoton microscopy: theory and experiment

Egner, A., Andresen, V., & Hell, S. W. (2002). Comparison of the axial resolution of practical Nipkow-disk confocal fluorescence microscopy with that of multifocal multiphoton microscopy: theory and experiment. Journal of Microscopy, 206, 24-32. Retrieved from http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2818.2002.01001.x/pdf.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F401-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-E58B-8
Genre: Journal Article

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 Creators:
Egner, A.1, Author              
Andresen, V.1, Author              
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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Free keywords: axial resolution; confocal microscopy; multifocal multiphoton microscopy
 Abstract: We compare the axial sectioning capability of multifocal confocal and multifocal multiphoton microscopy in theory and in experiment, with particular emphasis on the background arising from the cross-talk between adjacent imaging channels. We demonstrate that a time-multiplexed non-linear excitation microscope exhibits significantly less background and therefore a superior axial resolution as compared to a multifocal single- photon confocal. system. The background becomes irrelevant for thin (< 15 ?m) and sparse fluorescent samples, in which case the confocal parallelized system exhibits similar or slightly better sectioning behaviour due to its shorter excitation wavelength. Theoretical and experimental axial responses of practically implemented microscopes are given

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Language(s): eng - English
 Dates: 2002-04-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

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Title: Journal of Microscopy
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 206 Sequence Number: - Start / End Page: 24 - 32 Identifier: -