English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Fast 100 nm resolution three-dimensional microscope reveals structural plasticity of mitochondria in live yeast

Egner, A., Jakobs, S., & Hell, S. W. (2002). Fast 100 nm resolution three-dimensional microscope reveals structural plasticity of mitochondria in live yeast. Proceedings of the National Academy of Sciences of the United States of America, 99(6), 3370-3375. Retrieved from http://www.pnas.org/content/99/6/3370.full.pdf+html.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F40F-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-9E64-E
Genre: Journal Article

Files

show Files
hide Files
:
599665.pdf (Publisher version), 355KB
Name:
599665.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Egner, A.1, Author              
Jakobs, S.1, Author              
Hell, S. W.1, Author              
Affiliations:
1Research Group of High Resolution Optical Microscopy, MPI for Biophysical Chemistry, Max Planck Society, ou_578626              

Content

show
hide
Free keywords: -
 Abstract: By introducing beam-scanning multifocal multiphoton 4Pi- nfocal microscopy, we have attained fast fluorescence imaging of live cells with axial super resolution. Rapid scanning of up to 64 pairs of interfering high-angle fields and subsequent confocal detection enabled us to perform three to five times finer optical sectioning than confocal microscopy. In conjunction with nonlinear image estoration, we demonstrate, to our knowledge for the first time, three-dimensional imaging of live eukaryotic cells at an equilateral resolution of ~100 nm. This imaging mode allowed us to reveal the morphology and size of the green fluorescent protein-labeled mitochondrial compartment of live Saccharomyces cerevisiae (bakers’ yeast) growing on different carbon sources. Our studies show that itochondria of cells grown on medium containing glycerol as the only carbon source, as opposed to glucose-grown cells, exhibit a strongly branched tubular reticulum. We determine the average tubular diameter and find that it increases from 339 ± 5 nm to 360 ± 4 nm when changing from glucose to glycerol, that is, from a fermentable to a nonfermentable carbon source. Moreover, this change is associated with a 2.8-fold increase of the surface of the reticulum, resulting in an average increase in volume of the mitochondrial compartment by a factor of 3.0 ± 0.2.

Details

show
hide
Language(s): eng - English
 Dates: 2002-03-19
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Proceedings of the National Academy of Sciences of the United States of America
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 99 (6) Sequence Number: - Start / End Page: 3370 - 3375 Identifier: -