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  Separation of presynaptic and postsynaptic contributions to depression by covariance analysis of successive EPSCs at the calyx of Held synapse

Scheuss, V., Schneggenburger, R., & Neher, E. (2002). Separation of presynaptic and postsynaptic contributions to depression by covariance analysis of successive EPSCs at the calyx of Held synapse. Journal of Neuroscience, 22(3), 728-739. Retrieved from http://www.jneurosci.org/cgi/reprint/22/3/728.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F44B-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-279B-2
Genre: Journal Article

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 Creators:
Scheuss, V.1, Author              
Schneggenburger, R.2, Author              
Neher, E.1, Author              
Affiliations:
1Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578579              
2Research Group of Synaptic Dynamics and Modulation, MPI for Biophysical Chemistry, Max Planck Society, ou_578582              

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Free keywords: synaptic transmission; short-term plasticity; quantal analysis; covariance analysis; release site; release probability; quantal size
 Abstract: Synaptic short-term plasticity is considered to result from multiple cellular mechanisms, which may include presynaptic and postsynaptic contributions. We have recently developed a non- stationary EPSC fluctuation analysis (Scheuss and Neher, 2001) to estimate synaptic parameters and their transient changes during short-term synaptic plasticity. Extending the classical variance-mean approach, a short train of stimuli is applied repetitively, and the resulting EPSCs are analyzed for means, variances, and covariances. This provides estimates of the quantal size and quantal content for each EPSC in the train, and furthermore, an estimate of the number of release sites. The latter is less sensitive to heterogeneity in the release probability than that of the variance-mean approach. Here, we applied this analysis to the calyx of Held synapse in brainstem slices of young rats (postnatal day 8-10). We found significant negative covariance in the amplitude of successive EPSCs in a train. The analysis showed that the 10-fold depression in the EPSC amplitude during 100 Hz trains at elevated extracellular Ca2+ concentration resulted from a 2.5-fold reduction in quantal size caused by postsynaptic AMPA receptor desensitization and saturation, and a fourfold reduction in quantal content, which was partially relieved by application of cyclothiazide. The number of release sites estimated by covariance analysis was approximate to2000 and significantly larger than estimates from variance-mean parabolas.

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Language(s): eng - English
 Dates: 2002-02-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

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Title: Journal of Neuroscience
Source Genre: Journal
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Pages: - Volume / Issue: 22 (3) Sequence Number: - Start / End Page: 728 - 739 Identifier: -