English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Analysis of gene function in somatic mammalian cells using small interfering RNAs

Elbashir, S. M., Harborth, J., Weber, K., & Tuschl, T. (2002). Analysis of gene function in somatic mammalian cells using small interfering RNAs. Methods, 26(2), 199-213. Retrieved from http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WN5-45WGJF8-F-1P&_cdi=6953&_user=38661&_pii=S1046202302000233&_origin=search&_coverDate=02%2F28%2F2002&_sk=999739997&view=c&wchp=dGLbVtz-zSkWA&md5=c94da4baa1bbe2a59c130b0ea900a490&ie=/sdarticle.pdf.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F450-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-20DA-4
Genre: Journal Article

Files

show Files
hide Files
:
16474.pdf (Publisher version), 0B
Name:
16474.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Elbashir, S. M.1, Author              
Harborth, J.2, Author              
Weber, K.2, Author              
Tuschl, T.1, Author              
Affiliations:
1Research Group of Combinatorical Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578556              
2Department of Biochemistry and Cell Biology, MPI for biophysical chemistry, Max Planck Society, ou_578618              

Content

show
hide
Free keywords: RNA interference; small interfering RNA; posttranscriptional gene silencing; knockdown; double-stranded RNA
 Abstract: RNA interference (RNAi) is a highly conserved gene silencing mechanism that uses double-stranded RNA (dsRNA) as a signal to trigger the degradation of homologous mRNA. The mediators of sequence-specific mRNA degradation are 21- to 23-nt small interfering RNAs (siRNAs) generated by ribonuclease III cleavage from longer dsRNAs. Twenty-one-nuclcotide siRNA duplexes trigger specific gene silencing in mammalian somatic cells without activation of the unspecific interferon response. Here we provide a collection of protocols for siRNA-mediated knockdown of mammalian gene expression. Because of the robustness of the siRNA knockdown technology. genomewide analysis of human gene function in cultured cells has now become possible. (C) 2002 Elsevier Science (USA). All rights reserved.

Details

show
hide
Language(s): eng - English
 Dates: 2002-02
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Methods
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 26 (2) Sequence Number: - Start / End Page: 199 - 213 Identifier: -