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  Dual-color 4Pi-confocal microscopy with 3D-resolution in the 100 nm range

Kano, H., Jakobs, S., Nagorni, M., & Hell, S. W. (2002). Dual-color 4Pi-confocal microscopy with 3D-resolution in the 100 nm range. Ultramicroscopy, 90(2-3), 207-213. Retrieved from http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6TW1-443K184-1-G&_cdi=5549&_user=38661&_pii=S0304399101001322&_origin=search&_coverDate=02%2F28%2F2002&_sk=999099997&view=c&wchp=dGLbVtz-zSkzS&md5=d206ae2ac93f8cdbf9f687aab8c3205f&ie=/sdarticle.pdf.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F457-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-9E6C-D
Genre: Journal Article

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 Creators:
Kano, H., Author
Jakobs, S.1, Author              
Nagorni, M.1, Author              
Hell, S. W.1, Author              
Affiliations:
1Research Group of High Resolution Optical Microscopy, MPI for Biophysical Chemistry, Max Planck Society, ou_578626              

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 Abstract: We report the development of simultaneous two-color channel recording in 4Pi-confocal microscopy. A marked increase of spatial resolution over confocal microscopy becomes manifested in 4Pi-confocal three-dimensional (313) data stacks of dual- labeled objects. The fundamentally improved resolution is verified both with densely labeled fluorescence beads as well as with membrane labeled fixed Escherichia coli. The synergistic combination of dual-color 4Pi-confocal recording with image restoration results in dual-color imaging with a 3D resolution in the 100 nm range. (C) 2002 Elsevier Science B.V. All rights reserved

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Language(s): eng - English
 Dates: 2002-02
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: Ultramicroscopy
Source Genre: Journal
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Pages: - Volume / Issue: 90 (2-3) Sequence Number: - Start / End Page: 207 - 213 Identifier: -