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  Mass spectrometric Sequencing of individual peptides from combinatorial libraries via specific generation of chain- terminated sequences

Hoffmann, C., Blechschmidt, D., Kruger, R., Karas, M., & Griesinger, C. (2002). Mass spectrometric Sequencing of individual peptides from combinatorial libraries via specific generation of chain- terminated sequences. Journal of Combinatorial Chemistry, 4(1), 79-86. Retrieved from http://pubs.acs.org/doi/pdfplus/10.1021/cc010057x.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F4A2-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-E309-B
Genre: Journal Article

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 Creators:
Hoffmann, C.1, Author
Blechschmidt, D., Author
Kruger, R., Author
Karas, M., Author
Griesinger, C.2, Author              
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2Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

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 Abstract: Combinatorial peptide libraries are a versatile tool for drug discovery. On-bead assays identify reactive peptides by enzyme- catalyzed staining and, usually, sequencing by Edman degradation. Unfortunately, the latter method is expensive and time-consuming and requires free N termini of the peptides. A method of rapid and unambiguous peptide sequencing by utilizing synthesis-implemented generation of termination sequences with subsequent matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometric analysis is introduced here. The required capped sequences are determined and optimized for a specific peptide library by a computer algorithm implemented in the program Biblio. A total of 99.7% of the sequences of a heptapeptide library sample could be decoded utilizing a single bead for each spectrum. To synthesize these libraries, an optimized capping approach has been introduced.

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Language(s): eng - English
 Dates: 2002-01
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: Peer
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Title: Journal of Combinatorial Chemistry
Source Genre: Journal
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Pages: - Volume / Issue: 4 (1) Sequence Number: - Start / End Page: 79 - 86 Identifier: -