ausblenden:
Schlagwörter:
electron paramagnetic resonance; spin label; microwave saturation; saturation transfer; membrane protein; site directed spin labelling; spin-spin interaction
Zusammenfassung:
Non-linear electron spin resonance (EPR) techniques suitable for measuring proximity relationships in membranes are reviewed. These were developed during the past decade in order to measure changes sensitively in the spin-lattice relaxation time (T-1) of nitroxyl spin labels covalently attached to membrane lipids or proteins. In combination with paramagnetic quenching agents and double spin-labelling, the methods were further developed for distance measurements. Selected examples are given to illustrate different methods, and types of data obtained for both integral and peripheral membrane proteins.
