English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Progesterone inhibits insulin secretion by a membrane delimited, non-genomic action

Straub, S. G., Sharp, G. W. G., Meglasson, M. D., & De Souza, C. J. (2001). Progesterone inhibits insulin secretion by a membrane delimited, non-genomic action. Bioscience Reports, 21(5), 653-666. Retrieved from http://springerlink.metapress.com/content/6qtv771ker8kx03p/fulltext.pdf.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F52C-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-E33A-E
Genre: Journal Article

Files

show Files
hide Files
:
16510.pdf (Publisher version), 0B
 
File Permalink:
-
Name:
16510.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Biophysical Chemistry (Karl Friedrich Bonhoeffer Institute), Göttingen; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Straub, S. G.1, Author              
Sharp, G. W. G.1, Author              
Meglasson, M. D., Author
De Souza, C. J., Author
Affiliations:
1Department of Neurobiology, MPI for biophysical chemistry, Max Planck Society, ou_578595              

Content

show
hide
Free keywords: insulin secretion; pancreatic beta-cell; steroids; membrane effects
 Abstract: In rat islets, progesterone caused a prompt concentration- dependent inhibition of glucose-stimulated insulin release with an IC50 of 10 muM at 8.4 mM glucose. The inhibition was specific since both testosterone and 17beta-estradiol had no such effect. The degree of inhibition was similar in islets from male and female rats. The inhibition was not blocked in PTX-treated islets thus ruling out the Gi/Go proteins as mediators of the inhibition. Progesterone inhibited both glucose- and BayK-8644-stimulated insulin Secretion in HIT-T15 cells and the IC50 vs. 10 mM glucose was also 10 muM. There was no effect on intracellular cyclic AMP concentration in the presence 0.2 and 10 mM glucose. Progesterone decreased [Ca2+](i) under all conditions tested. The decrease in [Ca2+](i), was due to blockade of the L-type voltage-dependent Ca2+, channels. Under Ca2+-free conditions, progesterone did not inhibit the stimulation of insulin release due to the combination of glucose, phorbol ester and Forskolin. Thus blockade of Ca2+ entry appears to be the sole mechanism by which progesterone inhibits insulin release. As progesterone covalently linked to albumin had a similar inhibitory effect as progesterone itself, it is concluded that the steroid acts at the outer surface of the beta-cell plasma membrane. These effects would be classified as either AI or AIIb in the Mannheim classification of nongenomically initiated steroid actions.

Details

show
hide
Language(s): eng - English
 Dates: 2001-10
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Bioscience Reports
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 21 (5) Sequence Number: - Start / End Page: 653 - 666 Identifier: -