English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Water permeation across biological membranes: Mechanism and dynamics of Aquaporin-1 and GlpF.

de Groot, B. L., & Grubmueller, H. (2001). Water permeation across biological membranes: Mechanism and dynamics of Aquaporin-1 and GlpF. Science, 294, 2353-2357.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F5D8-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-E288-8
Genre: Journal Article

Files

show Files
hide Files
:
599912.pdf (Publisher version), 914KB
Name:
599912.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
de Groot, B. L.1, Author              
Grubmueller, H.1, Author              
Affiliations:
1Research Group of Theoretical Molecular Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578630              

Content

show
hide
Free keywords: -
 Abstract: Real time” molecular dynamics simulations of water permeation through human aquaporin-1 (AQP1) and the bacterial glycerol facilitator GlpF are presented. We obtained time-resolved, atomic-resolution models of the permeation mechanism across these highly selective membrane channels. Both proteins act as two-stage filters: Conserved fingerprint [asparagine-proline-alanine (NPA)] motifs form a selectivity-determining region; a second (aromatic/arginine) region is proposed to function as a proton filter. Hydrophobic regions near the NPA motifs are rate-limiting water barriers. In AQP1, a fine-tuned water dipole rotation during passage is essential for water selectivity. In GlpF, a glycerol-mediated “induced fit” gating motion is proposed to generate selectivity for glycerol over water.

Details

show
hide
Language(s): eng - English
 Dates: 2001
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 224305
Other: 34839
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Science
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 294 Sequence Number: - Start / End Page: 2353 - 2357 Identifier: -