Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  Estimating transmitter release rates from postsynaptic current fluctuations

Neher, E., & Sakaba, T. (2001). Estimating transmitter release rates from postsynaptic current fluctuations. The Journal of Neuroscience, 21(24), 9638-9654. doi:10.1523/JNEUROSCI.21-24-09638.2001.

Item is

Basisdaten

einblenden: ausblenden:
Genre: Zeitschriftenartikel

Dateien

einblenden: Dateien
ausblenden: Dateien
:
9638.full.pdf (Verlagsversion), 323KB
 
Datei-Permalink:
-
Name:
9638.full.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Eingeschränkt ( Max Planck Society (every institute); )
MIME-Typ / Prüfsumme:
application/pdf
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Neher, E.1, Autor           
Sakaba, T.2, Autor           
Affiliations:
1Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578579              
2Research Group of Biophysics of Synaptic Transmission, MPI for biophysical chemistry, Max Planck Society, ou_578583              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: A method is presented that allows one to estimate transmitter release rates from fluctuations of postsynaptic current records under conditions of stationary or slowly varying release. For experimental applications, we used the calyx of Held, a glutamatergic synapse, in which “residual current,” i.e., current attributable to residual glutamate in the synaptic cleft, is present. For a characterization of synaptic transmission, several postsynaptic parameters, such as the mean amplitude of the miniature postsynaptic current and an apparent single channel conductance, have to be known. These were obtained by evaluating variance and two more higher moments of the current fluctuations. In agreement with Fesce et al. (1986), we found both by simulations and by analyzing experimental records that high-pass filtering of postsynaptic currents renders the estimates remarkably tolerant against nonstationarities. We also found that release rates and postsynaptic parameters can be reliably obtained when release rates are low (∼10 events/msec). Furthermore, during a long-lasting stimulus, the transmitter release at the calyx of Held was found to decay to a low, stationary rate of 10 events/msec after depletion of the “releasable pool” of synaptic vesicles. This stationary release rate is compatible with the expected rate of recruitment of new vesicles to the release-ready pool of vesicles. MiniatureEPSC (mEPSC) size is estimated to be similar to the value of spontaneously occurring mEPSC under this condition.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2001-12-15
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1523/JNEUROSCI.21-24-09638.2001
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: The Journal of Neuroscience
  Andere : The Journal of Neuroscience: the Official Journal of the Society for Neuroscience
  Kurztitel : J. Neurosci.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Washington, DC : Society of Neuroscience
Seiten: - Band / Heft: 21 (24) Artikelnummer: - Start- / Endseite: 9638 - 9654 Identifikator: ISSN: 0270-6474
CoNE: https://pure.mpg.de/cone/journals/resource/954925502187_1