English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  The telomeric dG(GT)4G sequence can adopt a parallel-stranded double helical conformation

Shchyolkina, A. K., Borisova, O. F., Livshits, M. A., Klement, R., & Jovin, T. M. (2001). The telomeric dG(GT)4G sequence can adopt a parallel-stranded double helical conformation. Journal of Biomolecular Structure and Dynamics, 18, 493-503.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F713-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-0E73-7
Genre: Journal Article

Files

show Files
hide Files
:
600067.pdf (Publisher version), 845KB
Name:
600067.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Shchyolkina, A. K., Author
Borisova, O. F., Author
Livshits, M. A., Author
Klement, R.1, Author              
Jovin, T. M.2, Author              
Affiliations:
1Emeritus Group Laboratory of Cellular Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578629              
2Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

Content

show
hide
Free keywords: -
 Abstract: Oligonucleotides 3'-d(GTGTGTGTGG)-L-d(GGTGTGTGTG)-3' (hp-GT) and 3'-d(G44SG4STG4STG4STGG)-L-d(GGTGTGTGTG)-3' (hp-SGT), (L=(CH2CH2O)3), were shown by use of several optical techniques to form a novel parallel-stranded (ps) intramolecular double helix with purine-purine and pyrimidine-pyrimidine base pairing. The rotational relaxation time of hp-GT was similar to that of a 10-bp reference duplex, and the fraction of unpaired bases was determined to be ~7%, testifying to the formation of an intramolecular double helical hairpin by the sequence under the given experimental conditions. A quasi-two-state mode of ps-double helix formation was validated, yielding a helix-coil transition enthalpy of -135±5 kJ/mol. The G·G and T·T (or 4ST ·T) base pair configurations and conformational parameters of the double helix were derived with molecular modeling by force field techniques. Repetitive d(GT) sequences are abundant in telomers of different genomes and in the regulatory regions of genes. Thus, the observed conformational potential of the repetitive d(GT) sequence may be of importance in the regulation of cell processes.

Details

show
hide
Language(s): eng - English
 Dates: 2005-08-052001
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 236891
Other: 34686
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Biomolecular Structure and Dynamics
  Alternative Title : J. Biomol. Struct. Dyn.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 18 Sequence Number: - Start / End Page: 493 - 503 Identifier: -