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要旨:
Patch-clamp measurements of Ca2+ currents and membrane capacitance were performed on slices of mouse adrenal glands, using the perforated-patch configuration of the patch-clamp technique. These recording conditions are much closer to the in vivo situation than those used so far in most electrophysiological studies in adrenal chromaffin cells (isolated cells maintained in culture and whole-cell configuration). We observed profound discrepancies in the quantities of Ca2+channel subtypes (P-, Q-, N-, and L-type Ca2+channels) described for isolated mouse chromaffin cells maintained in culture. Differences with respect to previous studies may be attributable not only to culture conditions, but also to the patch-clamp configuration used. Our experiments revealed the presence of a Ca2+ channel subtype never before described in chromaffin cells, a toxin and dihydropyridine-resistant Ca2+ channel with fast inactivation kinetics, similar to the R-type Ca2+ channel described in neurons. This channel contributes 22% to the total Ca2+ current and controls 55% of the rapid secretory response evoked by short depolarizing pulses. Our results indicate that R-type Ca2+ channels are in close proximity with the exocytotic machinery to rapidly regulate the secretory process.
