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  Photophysics and optical switching in green fluorescent protein mutants.

Creemers, T. M. H., Lock, A. J., Subramaniam, V., Jovin, T. M., & Voelker, S. (2000). Photophysics and optical switching in green fluorescent protein mutants. Proceedings of the National Academy of Sciences USA, 97, 2974-2978.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F814-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-E7B1-F
Genre: Journal Article

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 Creators:
Creemers, T. M. H., Author
Lock, A. J., Author
Subramaniam, V.1, Author              
Jovin, T. M.1, Author              
Voelker, S., Author
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

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Free keywords: *Luminescent Proteins/ch [Chemistry]; Luminescent Proteins/ge [Genetics]; Mutagenesis; *Photochemistry; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Support, Non-U.S. Gov't; Structural basis; Crystal structure; Single molecules; Spectroscopy; Microscopy; Behavior
 Abstract: We demonstrate by using low-temperature high-resolution spectroscopy that red-shifted mutants of green fluorescent protein are photo-interconverted among three conformations and are, therefore, not photostable "one-color" systems as previously believed. From our experiments we have further derived the energy-level schemes governing the interconversion among the three forms. These results have significant implications for the molecular and cell biological applications of the green fluorescent protein family; for example, in fluorescence resonant energy transfer experiments, a change in "color" on irradiation may not necessarily be due to energy transfer but can also arise from a photo-induced conversion between conformers of the excited species.

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 Dates: 2005-08-172000
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 224272
Other: 23120
 Degree: -

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Title: Proceedings of the National Academy of Sciences USA
Source Genre: Journal
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Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 97 Sequence Number: - Start / End Page: 2974 - 2978 Identifier: -