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  EGFP and DsRed expressing cultures of Escherichia coli imaged by confocal, two-photon and fluorescence lifetime microscopy.

Jakobs, S., Subramaniam, V., Schönle, A., Jovin, T. M., & Hell, S. W. (2000). EGFP and DsRed expressing cultures of Escherichia coli imaged by confocal, two-photon and fluorescence lifetime microscopy. FEBS Letters, 479, 131-135.

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Jakobs, S.1, Autor           
Subramaniam, V.2, Autor           
Schönle, A.1, Autor           
Jovin, T. M.2, Autor           
Hell, S. W.1, Autor           
Affiliations:
1Research Group of High Resolution Optical Microscopy, MPI for Biophysical Chemistry, Max Planck Society, ou_578626              
2Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

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Schlagwörter: 2-photon excitation; Imaging microscopy; Protein; Gfp; Resolution; Mutants; Cells; green fluorescent protein; red fluorescent protein; confocal; Two-photon; fluorescence lifetime microscopy; Escherichia coli; E. coli
 Zusammenfassung: The green fluorescent protein (GFP) has become an invaluable marker for monitoring protein localization and gene expression in vivo. Recently a new red fluorescent protein (drFP583 or DsRed), isolated from tropical corals, has been described [Matz, M.V. et al, (1999) Nature Biotech, 17, 969-973]. With emission maxima at 509 and 583 nm respectively, EGFP and DsRed are suited for almost crossover free dual color labeling upon simultaneous excitation, We imaged mixed populations of Escherichia coli expressing either EGFP or DsRed by one-photon confocal and by two-photon microscopy, Both excitation modes proved to be suitable for imaging cells expressing either of the fluorescent proteins. DsRed had an extended maturation time and E, coli expressing this fluorescent protein were significantly smaller than those expressing EGFP, In aging bacterial cultures DsRed appeared to aggregate within the cells, accompanied by a strong reduction in its fluorescence lifetime as determined by fluorescence lifetime imaging microscopy.

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Sprache(n): eng - English
 Datum: 2000
 Publikationsstatus: Erschienen
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 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: eDoc: 226673
Anderer: 23125
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Titel: FEBS Letters
Genre der Quelle: Zeitschrift
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Seiten: - Band / Heft: 479 Artikelnummer: - Start- / Endseite: 131 - 135 Identifikator: -