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  Scanning force microscopy of the complexes of p53 core domain with supercoiled DNA

Jett, S. D., Cherny, D. I., Subramaniam, V., & Jovin, T. M. (2000). Scanning force microscopy of the complexes of p53 core domain with supercoiled DNA. Journal of Molecular Biology, 299, 585-592.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-F897-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-E91A-2
Genre: Journal Article

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 Creators:
Jett, S. D.1, Author              
Cherny, D. I.1, Author              
Subramaniam, V.1, Author              
Jovin, T. M.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

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Free keywords: DNA-protein complexes; p53; supercoiled DNA; cruciform; scanning force microscopy
 Abstract: We used scanning force microscopy to analyse the interaction of the core domain of the tumor suppressor protein p53 (p53CD, amino acid residues 94 to 312), with supercoiled DNA (scDNA) molecules. The complexes were attached to a mica substrate by the divalent cation spreading technique. p53CD bound to supercoiled plasmid pPGM1 bearing the consensus sequence 5'-AGACATGCCTAGACATGCCT-3' (p53CON) was imaged as a globular complex. Only one such complex was observed with each scDNA molecule. In contrast, binding to supercoiled pBluescript II SK(-) DNA (lacking the consensus sequence) resulted in the appearance of multiple, variable size complexes of various sizes on single DNA molecules. Addition of p53CD to scDNA containing a cruciform-forming (AT)(34) insert resulted in the binding of the protein exclusively at the cruciform. The data presented here suggest that p53CD can form stable specific and non-specific complexes with supercoiled DNA molecules, albeit of variable multimeric organization.

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Language(s): eng - English
 Dates: 2005-08-172000
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 226756
Other: 23126
 Degree: -

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Title: Journal of Molecular Biology
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 299 Sequence Number: - Start / End Page: 585 - 592 Identifier: -