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  Three photoconvertible forms of Green Fluorescent Protein identified by spectral hole-burning.

Creemers, T. M. H., Lock, A. J., Subramaniam, V., Jovin, T. M., & Voelker, S. (1999). Three photoconvertible forms of Green Fluorescent Protein identified by spectral hole-burning. Nature Structural and Molecular Biology, 6, 557-560.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-FA5C-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-8F7B-4
Genre: Journal Article

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600483.pdf (Publisher version), 887KB
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 Creators:
Creemers, T. M. H., Author
Lock, A. J., Author
Subramaniam, V.1, Author              
Jovin, T. M.1, Author              
Voelker, S., Author
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

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Free keywords: Structural basis; Spectroscopy
 Abstract: Several studies have led to the conclusion that, in the green fluorescent protein (GFP) of the jellyfish Aequorea victoria, a photoconversion involving excited-state proton transfer occurs from an A- to a B-form, while an intermediate I-form was held responsible for the green fluorescence. Here we have identified the I-form of wild-type GFP in absorption, located the 0-0 transitions of all three forms A, B and I, and determined vibrational frequencies of the ground and excited states. The intrinsically narrow 0-0 transitions are revealed by the wavelengths at which holes can be burnt. The pathways of photointerconversion are unraveled by excitation, emission and hole-burning spectroscopy. We present an energy-level scheme that has significant implications for GFP-mutants, which likewise can occur in the three photo-interconvertible forms.

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Language(s): eng - English
 Dates: 2005-08-151999
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 224271
Other: 11468
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Title: Nature Structural and Molecular Biology
Source Genre: Journal
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Pages: - Volume / Issue: 6 Sequence Number: - Start / End Page: 557 - 560 Identifier: -