English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Spectral imaging in a programmable array microscope by Hadamard transform fluorescence spectroscopy

Hanley, Q. S., Verveer, P. J., & Jovin, T. M. (1999). Spectral imaging in a programmable array microscope by Hadamard transform fluorescence spectroscopy. Applied Spectroscopy, 53(1), 1-10.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-FAAE-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-E818-3
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Hanley, Q. S.1, Author              
Verveer, P. J.1, Author              
Jovin, T. M.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

Content

show
hide
Free keywords: Acoustooptic tunable filter; Raman microscopy; Fourier-transform; Encoding mask; Spectrometry; Cells
 Abstract: We report the use of a thin-film transistor (TFT) twisted nematic liquid crystal spatial light modulator (SLM) for Hadamard transform two-dimensional spectral imaging in a fluorescence microscope. The liquid crystal SLM placed in the primary image plane of the microscope generates a set of spatial encoding masks defined by a cyclic S-matrix. The light passing through the mask is relayed by anamorphic optics to the entrance of an imaging spectrograph and detected with a charge-coupled device (CCD) camera. The SLM allows for the convenient generation of arbitrary masks without moving parts. The Hadamard transform approach transmits up to 50% of the light from the image field for multichannel detection, but is subject to transmission Losses in the SLM. The system allows the convenient acquisition of two-dimensional spectral images. We characterized and tested many of the parameters controlling both spatial and spectral resolution, and demonstrated the system in the analysis of both naturally fluorescing and stained biological samples.

Details

show
hide
Language(s): eng - English
 Dates: 2005-08-161999
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 226076
Other: 11471
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Applied Spectroscopy
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 53 (1) Sequence Number: - Start / End Page: 1 - 10 Identifier: -