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  Fluorescence resonance energy transfer detected by scanning near-field optical microscopy

Kirsch, A. K., Subramaniam, V., Jenei, A., & Jovin, T. M. (1999). Fluorescence resonance energy transfer detected by scanning near-field optical microscopy. Journal of Microscopy, 194(2/3), 448-454.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-FAF7-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-E75F-B
Genre: Journal Article

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 Creators:
Kirsch, A. K.1, Author              
Subramaniam, V.1, Author              
Jenei, A.2, Author
Jovin, T. M.2, Author
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              
2Max Planck Society, ou_persistent13              

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Free keywords: Animal; Energy Transfer; Fluorescence Polarization; Fluorescent Dyes/ch [Chemistry];Lectins/ch [Chemistry]; *Membrane Glycoproteins/an [Analysis]; Mice; *Microscopy, Confocal/mt [Methods]; Support, Non-U.S. Gov't; 3T3 Cells; Cell-surface proteins; fluorescence resonance energy transfer; photobleaching; protein-protein interactions; Scanning near-field optical microscopy; SNOM
 Abstract: Fluorescence resonance energy transfer (FRET) between excited fluorescent donor and acceptor molecules occurs via the Förster mechanism over a range of 1-10 nm. Because of the strong (sixth power) distance dependence of the signal, FRET has been used to assess the proximity of molecules in biological systems. We used a scanning near-field optical microscope (SNOM) operated in the shared-aperture mode using uncoated glass fibre tips to detect FRET between dye molecules embedded in polyvinyl alcohol films and bound to cell surfaces. FRET was detected by selective photobleaching of donor and acceptor fluorophores. We also present preliminary results on pixel-by-pixel energy transfer efficiency measurements using SNOM.

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Language(s): eng - English
 Dates: 2005-08-161999
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 226989
Other: 11474
 Degree: -

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Title: Journal of Microscopy
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 194 (2/3) Sequence Number: - Start / End Page: 448 - 454 Identifier: -