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  Fluorescence Correlation Microscopy (FCM) - Fluorescence Correlation Spectroscopy (FCS) taken into the cell

Brock, R., & Jovin, T. M. (1998). Fluorescence Correlation Microscopy (FCM) - Fluorescence Correlation Spectroscopy (FCS) taken into the cell. Cellular and Molecular Biology, 44, 847-856.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-FC70-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-E66F-F
Genre: Journal Article

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 Creators:
Brock, R.1, Author              
Jovin, T. M.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

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Free keywords: Fluorescence correlation spectroscopy; confocal fluorescence microscopy; fluorescence microscopy; Cell imaging; autofluorescence; molecular interaction
 Abstract: Confocal fluorescence correlation spectroscopy (FCS) and other confocal spectroscopic techniques are ideally suited for the analysis of molecular interactions at the subcellular level. However, one requires exact positioning in three dimensions within the cell. Our instrument integrates FCS with high sensitivity digital imaging microscopy and high precision positioning. We present first measurements of intracellular FCS, with specification of the instrumental requirements and methods of data analysis. We propose the term fluorescence correlation microscopy (FCM) for this extended modality of FCS.

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Language(s): eng - English
 Dates: 2005-07-081998
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 223394
Other: 622
 Degree: -

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Title: Cellular and Molecular Biology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 44 Sequence Number: - Start / End Page: 847 - 856 Identifier: -