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  Ultrastructural characterization of transcriptionally active chromatin by scanning force microscopy

Fritzsche, W., Spring, H., & Jovin, T. M. (1998). Ultrastructural characterization of transcriptionally active chromatin by scanning force microscopy. Probe Microscopy, 1, 225-232.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-FCC3-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-E622-C
Genre: Journal Article

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 Creators:
Fritzsche, W., Author
Spring, H., Author
Jovin, T. M.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

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Free keywords: Atomic force microscopy; Active genes; Miller spreading; Chromatin; DNA
 Abstract: Scanning force microscopy (SFM) was used for ultrastructural characterization of transcriptionally active chromatin. Newt oocyte chromatin was spread hypotonically, fixed and absorbed onto glass substrates prior to air drying. The well-known pattern of active genes was revealed in the topographic contrast of SFM. The measured dimensions for the DNA axis were up to14-16 nm (height) and 40-60 nm (width), for the RNA firbils 8-10 nm (height) and 30-50 nm (width). Due to their specific ultrastructure (i.e. gene length, polarity, repeat) ribosomal genes could be identified.

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Language(s): eng - English
 Dates: 2005-07-061998
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 224651
Other: 626
 Degree: -

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Title: Probe Microscopy
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 1 Sequence Number: - Start / End Page: 225 - 232 Identifier: -