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  Interferometric measurement of fluorescence excitation spectra

Hirschberg, J. G., Vereb, G., Meyer, C. K., Kirsch, A. K., Kohen, E., & Jovin, T. M. (1998). Interferometric measurement of fluorescence excitation spectra. Applied Optics, 37, 1953-1957.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-FCFF-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-E610-3
Genre: Journal Article

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Hirschberg, J. G., Author
Vereb, G.1, Author              
Meyer, C. K., Author
Kirsch, A. K.1, Author              
Kohen, E., Author
Jovin, T. M.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

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 Abstract: A Michelson interferometer has been adapted as an excitation source for fluorescence spectroscopy. A moving fringe pattern was generated by linear displacement of the movable mirror of the Michelson interferometer coupled to a xenon-arc lamp. This spectrally modulated source was monitored by a reference photomultiplier and used for exciting a Rhodamine B solution. The fluorescence emission at >645 nm was detected by a second photomultiplier. The two interferograms were acquired by a dual-channel digital oscilloscope, and their discrete Fourier transforms and corresponding power spectra were generated in a computer. The power spectrum of the emission signal represented the excitation spectrum, as was confirmed by comparison with the absorption spectrum of Rhodamine B. This optical arrangement is well suited for acquiring fluorescence excitation spectra in the optical microscopy of biological specimens.

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 Dates: 2005-07-061998
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
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 Identifiers: eDoc: 226580
Other: 631
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Title: Applied Optics
Source Genre: Journal
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Pages: - Volume / Issue: 37 Sequence Number: - Start / End Page: 1953 - 1957 Identifier: -