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  Distamycin-stabilized antiparallel-parallel-combination (APC) DNA

Shchyolkina, A. K., Minchenkova, L. E., Minyat, E. E., Khomyakova, Y. B., Ivanov, V. I., Klement, R., et al. (1998). Distamycin-stabilized antiparallel-parallel-combination (APC) DNA. Journal of Biomolecular Structure and Dynamics, 15, 823-839.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-FE05-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-0ADE-C
Genre: Journal Article

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 Creators:
Shchyolkina, A. K., Author
Minchenkova, L. E., Author
Minyat, E. E., Author
Khomyakova, Y. B., Author
Ivanov, V. I., Author
Klement, R.1, Author              
Jovin, T. M.2, Author              
Affiliations:
1Emeritus Group Laboratory of Cellular Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578629              
2Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

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Free keywords: Stranded duplex DNA; Base pairs; NMR; Netropsin; Complex; Purine; A.t.; Biochemistry & biophysics
 Abstract: The formation of Antiparallel-Parallel-Combination (APC) DNA, a liner duplex with a segment of parallel-stranded (ps) helix flanked by conventional B-DNA, was tested with a number of synthetic oligonucleotides. The groove-binding ligand distamycin A (DstA) was used to stabilize the ps segment comprising five AT base pairs. Two drug molecules bound per APC, one in each of the two equivalent grooves characteristic of ps-DNA. APC-DNA, reference molecules and their complexes with DstA were analysed by several methods: circular dichroism and absorption spectroscopy, thermal denaturation, chemical modification, and molecular modeling. The dye binding stoichiometry differed significantly due to inherent structural differences in the groove geometries of ps-DNA (trans base pairs, similar grooves) and conventional antiparallel-stranded (aps) B-DNA (cis base pairs, distinct major and minor grooves). The data support the existence of APC folding in solution.

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Language(s): eng - English
 Dates: 2005-08-051998
 Publication Status: Published in print
 Pages: -
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 Identifiers: eDoc: 236887
Other: 640
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Title: Journal of Biomolecular Structure and Dynamics
  Alternative Title : J. Biomol. Struct. Dyn.
Source Genre: Journal
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Pages: - Volume / Issue: 15 Sequence Number: - Start / End Page: 823 - 839 Identifier: -