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  Multiple Forms of Endocytosis In Bovine Adrenal Chromaffin Cells

Smith, C., & Neher, E. (1997). Multiple Forms of Endocytosis In Bovine Adrenal Chromaffin Cells. The Journal of Cell Biology: JCB, 139(4), 885-894. doi:10.1083/jcb.139.4.885.

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Smith, C.1, Author           
Neher, E.2, Author           
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1Abteilung Neurobiologie, MPI for biophysical chemistry, Max Planck Society, ou_578620              
2Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578579              

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 Abstract: We studied endocytosis in chromaffin cells with both perforated patch and whole cell configurations of the patch clamp technique using cell capacitance measurements in combination with amperometric catecholamine detection. We found that chromaffin cells exhibit two relatively rapid, kinetically distinct forms of stimulus-coupled endocytosis. A more prevalent “compensatory” retrieval occurs reproducibly after stimulation, recovering an approximately equivalent amount of membrane as added through the immediately preceding exocytosis. Membrane is retrieved through compensatory endocytosis at an initial rate of ∼6 fF/s. Compensatory endocytotic activity vanishes within a few minutes in the whole cell configuration. A second form of triggered membrane retrieval, termed “excess” retrieval, occurs only above a certain stimulus threshold and proceeds at a faster initial rate of ∼248 fF/s. It typically undershoots the capacitance value preceding the stimulus, and its magnitude has no clear relationship to the amount of membrane added through the immediately preceding exocytotic event. Excess endocytotic activity persists in the whole cell configuration. Thus, two kinetically distinct forms of endocytosis coexist in intact cells during perforated patch recording. Both are fast enough to retrieve membrane after exocytosis within a few seconds. We argue that the slower one, termed compensatory endocytosis, exhibits properties that make it the most likely mechanism for membrane recycling during normal secretory activity.

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Language(s): eng - English
 Dates: 1997-11-17
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1083/jcb.139.4.885
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Title: The Journal of Cell Biology : JCB
  Other : J. Cell Biol.
Source Genre: Journal
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Publ. Info: New York, NY : Rockefeller Institute Press
Pages: - Volume / Issue: 139 (4) Sequence Number: - Start / End Page: 885 - 894 Identifier: ISSN: 0021-9525
CoNE: https://pure.mpg.de/cone/journals/resource/991042742946024