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  Alteration of Ca2+ Dependence of Neurotransmitter Release by Disruption of Ca2+ Channel/Syntaxin Interaction

Rettig, J., Heinemann, C., Ashery, U., Sheng, Z. H., Yokoyama, C. T., Catterall, W. A., et al. (1997). Alteration of Ca2+ Dependence of Neurotransmitter Release by Disruption of Ca2+ Channel/Syntaxin Interaction. The Journal of Neuroscience, 17(17), 6647-6656. doi:10.1523/JNEUROSCI.17-17-06647.1997.

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Genre: Journal Article
Other : Alteration of Ca2+ Dependence of Neurotransmitter Release by Disruption of Ca2+ Channel/Syntaxin Interaction
Other : Alteration of Ca++ Dependence of Neurotransmitter Release by Disruption of Ca++ Channel/Syntaxin Interaction

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6647.full.pdf (Publisher version), 512KB
 
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 Creators:
Rettig, J.1, Author           
Heinemann, C.1, Author           
Ashery, U.1, Author           
Sheng, Z. H., Author
Yokoyama, C. T., Author
Catterall, W. A., Author
Neher, E.1, Author           
Affiliations:
1Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578579              

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Free keywords: neuromuscular junction; Ca2+ dependence; synaptic transmission; calcium channel; synprint; syntaxin
 Abstract: Presynaptic N-type calcium channels interact with syntaxin and synaptosome-associated protein of 25 kDa (SNAP-25) through a binding site in the intracellular loop connecting domains II and III of the α1 subunit. This binding region was loaded into embryonic spinal neurons of Xenopus by early blastomere injection. After culturing, synaptic transmission of peptide-loaded and control cells was compared by measuring postsynaptic responses under different external Ca2+ concentrations. The relative transmitter release of injected neurons was reduced by ∼25% at physiological Ca2+ concentration, whereas injection of the corresponding region of the L-type Ca2+channel had virtually no effect. When applied to a theoretical model, these results imply that 70% of the formerly linked vesicles have been uncoupled after action of the peptide. Our data suggest that severing the physical interaction between presynaptic calcium channels and synaptic proteins will not prevent synaptic transmission at this synapse but will make it less efficient by shifting its Ca2+ dependence to higher values.

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Language(s): eng - English
 Dates: 1997-09-01
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
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Title: The Journal of Neuroscience
  Other : The Journal of Neuroscience: the Official Journal of the Society for Neuroscience
  Abbreviation : J. Neurosci.
Source Genre: Journal
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Publ. Info: Washington, DC : Society of Neuroscience
Pages: - Volume / Issue: 17 (17) Sequence Number: - Start / End Page: 6647 - 6656 Identifier: ISSN: 0270-6474
CoNE: https://pure.mpg.de/cone/journals/resource/954925502187_1