English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Scanning near-field optical microscopy and microspectroscopy of green fluorescent protein in intact Escherichia coli bacteria

Subramaniam, V., Kirsch, A. K., Rivera-Pomar, R., & Jovin, T. M. (1997). Scanning near-field optical microscopy and microspectroscopy of green fluorescent protein in intact Escherichia coli bacteria. Journal of Fluorescence, 7(4), 381-385.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-0068-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-EA0B-B
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Subramaniam, V.1, Author              
Kirsch, A. K.1, Author              
Rivera-Pomar, R.2, Author              
Jovin, T. M.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              
2Department of Cellular Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578576              

Content

show
hide
Free keywords: Near-field; Scanning near-field optical microscopy; fluorescence microscopy; three dimensional imaging
 Abstract: Scanning near-field optical microscopy (SNOM) yields high-resolution topographic and optical information and constitutes an important new technique for visualizing biological systems. By coupling a spectrograph to a near-field microscope, we have been able to perdorem microspectroscopic measurements with a spatial resolution greatly exceeding that of the conventional optical microscope. Here we present SNO images of Escherischia coli bacteria expressing a mutant green fluorescent protein (GFP), an important reporter molecule in the cell, developmental, and molecular biology. Near-field emission spectra confirm that the fluorescence detected by SNOM arises from bacterially expressed GFP molecules.

Details

show
hide
Language(s): eng - English
 Dates: 2005-08-151997
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 237784
Other: 643
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Fluorescence
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 7 (4) Sequence Number: - Start / End Page: 381 - 385 Identifier: -