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  Imaging the electrocyte of Torpedo marmorata by scanning force microscopy

Pietrasanta, L. I., Schaper, A., Fox, G. Q., Barrantes, F. J., & Jovin, T. M. (1996). Imaging the electrocyte of Torpedo marmorata by scanning force microscopy. Scanning Microscopy, 10, 963-974.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-0216-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-EC78-7
Genre: Journal Article

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Pietrasanta, L. I.1, Author              
Schaper, A.1, Author              
Fox, G. Q., Author
Barrantes, F. J., Author
Jovin, T. M.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

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 Abstract: Scanning force microscopy (SFM) and scanning electron microscopy (SEM) were used to examine the tissue structure of the electric organ of Torpedo marmorata in air and in liquid after applying fracturing and cryosectioning techniques and chemical fixation. The electric organ is organized in columns of stacked electrocytes, arranged in a honeycomb pattern. The columns were cut along a plane normal to the cell stack and thin sections were transferred to polylysine coated glass cover slips. The polarity of the electrocytes was made apparent by immunofluorescence microscopy directed to different domains of the acetylcholine receptor (AChR), thus revealing the innervated face of the cell. SFM and SEM both showed cell surfaces to be overlaid by a network of collagen fibers by their characteristic banding pattern with ~64 nm periodicity and ~2.5 nm corrugation amplitude. In liquid, significantly lower structural resolution was achieved by SFM, probably due to sample elasticity.

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Language(s): eng - English
 Dates: 2005-08-151996
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 233611
Other: 636
 Degree: -

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Title: Scanning Microscopy
Source Genre: Journal
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Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 10 Sequence Number: - Start / End Page: 963 - 974 Identifier: -