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  Time course of Ca2+ concentration triggering exocytosis in neuroendocrine cells

Chow, R. H., Klingauf, J., & Neher, E. (1994). Time course of Ca2+ concentration triggering exocytosis in neuroendocrine cells. Proceedings of the National Academy of Sciences of the United States of America, 91(26), 12765-12769. doi:10.1073/pnas.91.26.12765.

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Genre: Zeitschriftenartikel
Andere : Time course of Ca2+ concentration triggering exocytosis in neuroendocrine cells

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 Urheber:
Chow, R. H.1, Autor           
Klingauf, J.2, Autor           
Neher, E.1, Autor           
Affiliations:
1Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578579              
2Research Group of Microscopy of Synaptic Transmission, MPI for biophysical chemistry, Max Planck Society, ou_578580              

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Schlagwörter: MICRODOMAIN; CALCIUM DOMAIN; CHROMAFFIN; CAPACITANCE; AMPEROMETRY
 Zusammenfassung: We have used the secretory response of chromaffin cells to estimate the submembrane intracellular Ca2+ concentration ([Ca2+]i) "seen" by secretory granules during short depolarizations. The rate of secretion during a depolarization was assessed by combining the electrochemical method of amperometry and electrical capacitance measurements. The rate was then related to [Ca2+]i based on a previous characterization of how Ca2+ affects the dynamics of vesicle priming and fusion in chromaffin cells [Heinemann, C., Chow, R. H., Neher, E. & Zucker, R. S. (1994) Biophys. J. 67, in press]. Calculated [Ca2+]i rose during the depolarization to a peak of < 10 microM, then decayed over tens of milliseconds. In synapses, vesicles are presumed to be located within nanometers of Ca2+ channels where [Ca2+]i is believed to rise in only microseconds to near steady-state levels of hundreds of micromolar. Channel closure should lead to a decrease in [Ca2+]i also in microseconds. Our findings of the slower time course and the lower peak [Ca2+]i suggest that in chromaffin cells, unlike synapses, Ca2+ channels and vesicles are not strictly colocalized. This idea is consistent with previously published data on dense-core vesicle secretion from diverse cell types.

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Sprache(n): eng - English
 Datum: 1994-12-20
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1073/pnas.91.26.12765
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Titel: Proceedings of the National Academy of Sciences of the United States of America
  Andere : PNAS
  Andere : Proceedings of the National Academy of Sciences of the USA
  Kurztitel : Proc. Natl. Acad. Sci. U. S. A.
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Washington, D.C. : National Academy of Sciences
Seiten: - Band / Heft: 91 (26) Artikelnummer: - Start- / Endseite: 12765 - 12769 Identifikator: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230