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  Kinetics of the secretory response in bovine chromaffin cells following flash photolysis of caged Ca2+

Heinemann, C., Chow, R. H., Neher, E., & Zucker, R. S. (1994). Kinetics of the secretory response in bovine chromaffin cells following flash photolysis of caged Ca2+. Biophysical Journal, 67(6), 2546-2557. doi:10.1016/S0006-3495(94)80744-1.

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Genre: Journal Article
Other : Kinetics of the secretory response in bovine chromaffin cells following flash photolysis of caged Ca2+

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Heinemann, C.1, Author           
Chow, R. H.1, Author           
Neher, E.1, Author           
Zucker, R. S., Author
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1Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578579              

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 Abstract: The kinetics of the secretory response in bovine chromaffin cells following flash photolysis of caged Ca2+ were studied by capacitance (Cm) measurements with millisecond time resolution. After elevation of the internal Ca2+ concentration ([Ca2+]i), Cm rises rapidly with one or more exponentials. The time constant of the fastest component decreases for higher [Ca2+]i (range 3–600 microM) over three orders of magnitude before it saturates at approximately 1 ms. The corresponding maximal rates of secretion can be as fast as 100,000 fF/s or 40,000 vesicles/s. There is a Ca(2+)-dependent delay before Cm rises, which may reflect the kinetics of multiple Ca2+ ions binding to the secretory apparatus. The initial rise in Cm is described by models containing a sequence of two to four single Ca(2+)-binding steps followed by a rate-limiting exocytosis step. The predicted Ca2+ dissociation constant (Kd) of a single Ca(2+)-binding site is between 7 and 21 microM. At [Ca2+]i > 30 microM clear indications of a fast endocytotic process complicate the analysis of the secretory response.

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Language(s): eng - English
 Dates: 1994-12
 Publication Status: Issued
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 Rev. Type: Peer
 Identifiers: DOI: 10.1016/S0006-3495(94)80744-1
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Title: Biophysical Journal
  Other : Biophys. J.
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 67 (6) Sequence Number: - Start / End Page: 2546 - 2557 Identifier: ISSN: 0006-3495
CoNE: https://pure.mpg.de/cone/journals/resource/954925385117