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  Delay in vesicle fusion revealed by electrochemical monitoring of single secretory events in adrenal chromaffin cells

Chow, R. H., von Rueden, L., & Neher, E. (1992). Delay in vesicle fusion revealed by electrochemical monitoring of single secretory events in adrenal chromaffin cells. Nature, 356(6364), 60-63. doi:10.1038/356060a0.

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Chow, R. H.1, Author           
von Rueden, L.1, Author           
Neher, E.1, Author           
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1Department of Membrane Biophysics, MPI for biophysical chemistry, Max Planck Society, ou_578579              

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 Abstract: I N synapses, a rise in presynaptic intracellular calcium leads to secretory vesicle fusion in less than a millisecond, as indicated by the short delay from excitation to postsynaptic signal1–4. In non-synaptic secretory cells, studies at high time resolution have been limited by the lack of a detector as fast and sensitive as the postsynaptic membrane. Electrochemical methods may be sensitive enough to detect catecholamines released from single vesicles5,6. Here, we show that under voltage-clamp conditions, stochastically occurring signals can be recorded from adrenal chromaffin cells using a carbon-fibre electrode as an electrochemical detector. These signals obey statistics characteristic for quantal release; however, in contrast to neuronal transmitter release, secretion occurs with a significant delay after short step depolarizations. Furthermore, we identify a pedestal or 'foot' at the onset of unitary events which may represent the slow leak of catecholamine molecules out of a narrow 'fusion pore' before the pore dilates for complete exocytosis.

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Language(s): eng - English
 Dates: 1992-03-05
 Publication Status: Issued
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/356060a0
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Title: Nature
  Abbreviation : Nature
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 356 (6364) Sequence Number: - Start / End Page: 60 - 63 Identifier: ISSN: 0028-0836
CoNE: https://pure.mpg.de/cone/journals/resource/954925427238