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  Fluid phase connectivity in an isomorphous, two-component, two-phase phosphatidylcholine bilayer.

Vaz, W. L. C., Melo, E. C. C., & Thompson, T. E. (1990). Fluid phase connectivity in an isomorphous, two-component, two-phase phosphatidylcholine bilayer. Biophysical Journal, 58(1), 273-275. doi:10.1016/S0006-3495(90)82373-0.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-0D9C-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-36B6-E
Genre: Journal Article

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602963.pdf (Publisher version), 420KB
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Vaz, W. L. C.1, Author              
Melo, E. C. C.1, Author              
Thompson, T. E.2, Author
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              
2Max Planck Society, ou_persistent13              

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 Abstract: Two-dimensional fluid phase connectivity is examined in mixed bilayers of dimyristoyl phosphatidylcholine and dipalmitoyl phosphatidylcholine as a function of composition and temperature at constant pressure using fluorescence recovery after photobleaching (FRAP). These isomorphous phospholipid mixtures exhibit nearly ideal mixing behavior. Dilauroyl phosphatidylethanolamine covalently linked through its amino function to NBD is used as the fluorescent probe in this study. These studies show the line of connectivity to be coincident with the line connecting the midpoints of all tie lines in the two-phase region of the phase diagram.

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Language(s): eng - English
 Dates: 1990-07
 Publication Status: Published in print
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 Rev. Method: Peer
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Title: Biophysical Journal
Source Genre: Journal
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Pages: - Volume / Issue: 58 (1) Sequence Number: - Start / End Page: 273 - 275 Identifier: ISSN: 0006-3495