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  Characterization by cDNA cloning of the mRNA for seminalplasmin, the major basic protein of bull semen.

Wagner, S., Freudenstein, J., & Scheit, K. H. (1990). Characterization by cDNA cloning of the mRNA for seminalplasmin, the major basic protein of bull semen. DNA and Cell Biology, 9(6), 437-442. doi:10.1089/dna.1990.9.437.

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 Creators:
Wagner, S.1, Author
Freudenstein, J.2, Author              
Scheit, K. H.2, Author              
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1Max Planck Society, ou_persistent13              
2Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

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 Abstract: A cDNA library derived from poly(A)+RNA of bull seminal vesicle tissue was screened with synthetic DNA probes specific for seminalplasmin (SAP), the major basic protein of bull semen. From a number of positive clones, pBSV12, containing a 577-bp insert, was identified and sequenced. The derived amino acid sequence comprises the known amino acid sequence of SAP with an amino terminal representing a putative signal sequence; at the carboxyl terminus the sequence contains an additional lysine residue. Present experimental data do not distinguish between two potential SAP precursor molecules, each starting with a methionine residue and differing by 10 amino acid residues in the leader peptide. Comparative Northern analysis reveals a SAP-specific mRNA of 700 bp, which lacks RNA from bovine testis as well as from seminal vesicle tissue of a bull calf; hence, expression of the SAP gene appears to be under androgen and/or developmental control. Southern analysis indicates that one gene appears to specify SAP. SAP-like DNA sequences were detected in ovine and porcine genomic DNA.

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Language(s): eng - English
 Dates: 1990-07
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1089/dna.1990.9.437
ISI: A1990DR29500005
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Title: DNA and Cell Biology
Source Genre: Journal
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Pages: - Volume / Issue: 9 (6) Sequence Number: - Start / End Page: 437 - 442 Identifier: ISSN: 1044-5498