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  A parallel stranded linear DNA duplex incorporating dG.dC base pairs.

Rippe, K., Ramsing, N. B., Klement, R., & Jovin, T. M. (1990). A parallel stranded linear DNA duplex incorporating dG.dC base pairs. Journal of Biomolecular Structure & Dynamics, 7(6), 1199-1209. doi:10.1080/07391102.1990.10508559.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0013-0DC2-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-2FE4-A
Genre: Journal Article

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 Creators:
Rippe, K.1, Author              
Ramsing, N. B., Author
Klement, R.2, Author              
Jovin, T. M.1, Author              
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              
2Emeritus Group Laboratory of Cellular Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578629              

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 Abstract: DNA oligonucleotides with appropriately designed complementary sequences can form a duplex in which the two strands are paired in a parallel orientation and not in the conventional antiparallel double helix of B-DNA. All parallel stranded (ps) molecules reported to date have consisted exclusively of dA.dT base pairs. We have substituted four dA.dT base pairs of a 25-nt parallel stranded linear duplex (ps-D1.D2) with dG.dC base pairs. The two strands still adopt a duplex structure with the characteristic spectroscopic properties of the ps conformation but with a reduced thermodynamic stability. Thus, the melting temperature of the ps duplex with four dG.dC base pairs (ps-D5.D6) is 10-16 degrees C lower and the van't Hoff enthalpy difference delta HvH for the helix-coil transition is reduced by 20% (in NaCl) and 10% (in MgCl2) compared to that of ps-D1.D2. Based on energy minimizations of a ps-[d(T5GA5).d(A5CT5)] duplex using force field calculations we propose a model for the conformation of a trans dG.dC base pair in a ps helix

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Language(s): eng - English
 Dates: 1990-06
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: Peer
 Degree: -

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Title: Journal of Biomolecular Structure & Dynamics
Source Genre: Journal
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Pages: - Volume / Issue: 7 (6) Sequence Number: - Start / End Page: 1199 - 1209 Identifier: ISSN: 0739-1102